Applications

Stem Cells

Regenerative therapy through cell therapy is often combined with gene therapy. This gene therapy consists of modifying the DNA of the target cells.


Classically, viruses capable of inserting new genetic information into the genome of the cells have been used, but nowadays, a much more advanced and refined genetic modification system known as CRISPR-Cas9 or Prime Editing, where different enzymes play an important role in the process of exchanging genetic material, opening up the possibility of inserting, eliminating or correcting DNA sequences in a much more controlled manner.

Bioprocess

Strategies

There are two strategies in regenerative therapy:

· Autologous: when only the patient’s own cells are used.
· Allogeneic: when the recipient of the cells is not the donor himself.

Applicable areas

– Conditioned media: culture media in which the cells have secreted certain molecules. In this aspect, the use of stem cells is not only restricted to cells of animal origin but jumps to cultures with plant stem cells, in which an infinite horizon is opening up in the world of phytocomplexes.


Plant stem cells secrete different molecules that work in synergy and recently, the fraction of polyphenols recovered from these phytocomplexes is showing enormous potential in biomedicine and cosmetics. This new form of cultivation is more economical and environmentally friendly than traditional plantations. It is also simpler and cheaper than that of animal origin and the scalability in STR bioreactors does not differ from that of cultures of microbial origin. On the other hand, downstream processes usually require more complex equipment that allows a greater effectiveness of cell lysis, since cells tend to form very compact aggregates between them, and in this case FFT can be the ideal solution to ensure the performance of the process.


– Food industry: artificial meat is achieved by culturing muscle stem cells on matrix surfaces or carriers. This possibility is in the sights of many companies that are betting on the leap to more ecological cultivation of any animal organ, although for the moment it needs strong legislative and economic backing to prosper adequately.