Exploring Recombinant Protein Production with TECNIC'S Biotech Solutions
In the dynamic realm of biotechnology, recombinant proteins production has become one of the major techniques to produce highly desired protein products for a large range of life-science applications, such as medical research and pharmaceuticals.
In the past, the major way to obtain a specific protein was to isolate it from a natural source, which was generally inefficient and time-consuming. However, thanks to recent advances in genetic engineering and recombinant molecule biological techniques, the biotech landscape has undergone a transformative shift, making it possible to express custom recombinant proteins within dedicated expression systems, such as bacteria, yeast, insect cells, algal systems and mammalian cells.
This biotechnological innovation has become not only fundamental to biomedicine and pharmaceutical bioprocesses, but also to other biotech applications, offering innovative solutions to global challenges. As this technology continues to evolve, its potential promises to further revolutionize our approach to many fields of life sciences.
At Tecnic we recognize the pivotal role that recombinant proteins play in biotechnology, and for that reason, we ensure that our state-of-the-art equipment is meticulously designed to elevate and optimize its production with efficiency, precision and scalability. Our comprehensive range of bioreactors empowers researchers and industry manufacturers to achieve enhanced yield, and our TFF systems allow for easy and automatic purification of the desired product.
Explore the infinite possibilities with Tecnic and choose us to work with a partner committed to catalyze breakthroughs in production efficiency through our innovation solutions.
What are recombinant proteins?
If proteins are the workhorse in biological systems, driving most cell bioprocesses, recombinant proteins are those expressed from a recombinant DNA gene, which has been genetically engineered to be cloned into an expression vector. Once inserted into the host organism, the vector supports the recombinant protein expression through transcription and subsequent RNA translation within the desired host cells.
The host organism (for exemple E. coli), now genetically modified, essentially becomes a specialized factory for the production of the recombinant protein. This biotechnology method proves invaluable, enabling the production of proteins that may be otherwise challenging to obtain in large quantities or with specific custom modifications using traditional methods. Recombinant proteins bring a huge set of benefits to the life sciences, as they allow for the production of highly desired protein products with high yields in large-scale productions
Moreover, by using genetic engineering methodologies to modify the DNA sequence encoding a specific protein, it is possible to induce alterations in its structure, function, or other inherent properties, serving as the means to engineer recombinant proteins with tailored characteristics to meet diverse requirements. This inherent adaptability for custom protein production extends beyond the creation of novel proteins for distinct purposes, but it also encompasses the enhancement of existing ones.
Recombinant protein uses
There are several types of recombinant proteins, each with distinctive fields of applications, but most of them have become indispensable in various medical fields.
For example, in the pharmaceutical industry, the production of recombinant proteins is paramount for developing vaccines and therapeutic agents, as they often serve as the active pharmaceutical ingredient in biopharmaceuticals. For instance, recombinant antibodies such as monoclonal antibodies or immunoglobulin antibodies and other advanced therapeutic modalities are commonly produced using this custom-expressing protein system. Also, the precision and scalability of recombinant protein technology have revolutionized drug development, enabling the production of high-quality, standardized therapeutic proteins such as insulin, growth factors or some hormones.
In the field of diagnostics, as the performance and availability of recombinant proteins increase, they are used to developing more highly sensitive and specific tests for various diseases. For example, even if a virus cannot be cultured, provided its gene sequence, it is possible to rapidly respond to emerging viruses or new viral strains with an ELISA test that is produced by using recombinant proteins of the specific virus.
Crucial role in research
In research, they have been crucial for the study of protein function and interactions, which is essential for understanding biological processes and disease mechanisms. Furthermore, sophisticated techniques like surface plasmon resonance are employed to analyze their interactions, contributing to a deeper understanding of their biological roles and gaining insights into the functional properties of these proteins. For example, expressing proteins in E. coli has allowed biomedical research to study intricate biochemical pathways and decipher the molecular mechanisms underlying various biological processes. Moreover, using genome editing tools like CRISPR CAS9 or Cre-lox technology, the use of recombinant proteins of genes such as Rag 1 and Rag 2 or the CNTF gene has provided valuable insight into the regulation of gene expression, signal transduction pathways, and the intricate interplay between different cellular components.
Furthermore, recombinant protein uses in technology are not only fundamental to medicine but also to other fields like agriculture and new food technology, offering promising solutions to complex challenges in the biotech industry. As this technology continues to evolve, its potential for future innovations promises to further revolutionize our approach to life sciences, both in research and industry applications, and in TECNIC we want to be part of the newest advancement solutions to evolve together.
Challenges in recombinant protein production
This biotechnology requires an enormous amount of resources and know-how, and failures in manufacturing can have severe consequences as well as financial drawbacks. Its common challenges encompass issues related to efficiency, purity and scalability and demand careful consideration and sophisticated solutions for optimal outcomes.
The Importance of TFF in the Production of Recombinant Proteins
In order to produce high-purity recombinant proteins with elevated yields and consistent results, it is imperative to work with the best equipment, whether you are producing therapeutic antibodies for research purposes or manufacturing large enzymes for industry. As the premier solution, TECNIC Bioreactors ⇀ stand out for upstream recombinant protein bioprocessing production, addressing the key challenges with expertise at every step, from research up to large-scale industry manufacturing.
Allow us to provide precise control over all environmental parameters, optimizing the best cellular or microbial conditions for protein yield while minimizing resource and time utilization. Robust monitoring capabilities ensure maximal efficiency in bioprocess and the TECNIC automatic control unit of eLAB, ePILOT and ePROD ⇀ , with an industrial PLC, enables you to fine-tune your culture conditions to achieve reproducible and consistent results.
Once the final stage of the culture is achieved, it is primordial to obtain a good end-product result. The advanced downstream Tangential Flow Filtration of TECNIC ⇀ effectively separates the target protein from impurities and contaminants, ensuring a high level of purity with high flow rates. Also, thanks to its fully automatic system and variety of operational options, eLAB TFF ⇀ , ePILOT TFF ⇀ and ePROD TFF ⇀ contribute to the final purity of your overall bioprocess.
Finally, given the inherent scalability of our equipment, TECNIC's bioreactors and tangential system adaptability ensures the reliability and efficiency required to meet the end demand for high-quality recombinant proteins. So, whether you are engaged in pioneering research or driving industrial-scale production, TECNIC's commitment is the solution to evolve together.
Success stories and applications
While we cannot provide specific details due to confidentiality, there have been numerous instances where TECNIC's equipment has significantly enhanced the production of recombinant proteins. Our clients placed their trust in us, and the success of the provided TECNIC solutions underscores our excellence and commitment to evolve together.
For example, in the realm of new food research, one of our clients aimed to produce egg highly functional proteins using a non-animal source. While they already possessed the technology and the knowledge, they encountered several challenges during Scale-Up production. Leveraging the TECNIC eLAB Advanced Bioreactor, they were able to optimize the expression system, leading to a significant increase in both yield and purity. Also, the versatility of this equipment, coupled with its scalability features, allowed them to work seamlessly with multiple vessels for different stages of the production process. As a result, our client achieved their production goals with enhanced efficiency and product quality, achieving optimal outcomes for their project.
Our commitment to our clients’ projects ensures that we stay updated on any developments or additional assistance that may be required, and for now, we are still providing ongoing support and staying actively engaged with this client project.
In the pharmaceutical sector, TECNIC is used to collaborate with companies that are focused on large-scale production of biotech pharma products such as monoclonal antibodies ⇀, anticancer drugs, cell therapies ⇀, and more.
These cutting-edge pharmaceutical and biotechnology products often require the use of bioreactors for upstream processes and downstream tangential filtration systems to ensure optimal yield, purity, and scalability.
For instance, TECNIC is deeply involved in the large-scale manufacturing of an innovative therapeutic antibiotic designed for livestock. In this specific biotechnological endeavour, our ePROD equipment plays a pivotal role, providing the precision and perfect control of the bioprocess to ensure the high yields expected of this project. Subsequently, our ePROD TFF effectively separates the target peptide from the media culture and other impurities. At present, the overall project attains a high level of purity coupled with impressive yield rates, a testament to our commitment to client success by delivering optimal solutions at every stage of the bioprocess.
Explore our complete range of equipment
Explore the forefront of recombinant protein with our advanced equipment. Our bioreactors are designed for diverse applications, ensuring efficiency and quality. Discover our full range of biotechnology solutions, including Bioreactors, Tangential Flow Filtration systems, and more. Visit our pages for Bioreactors ⇀, Tangential Flow Filtration ⇀, and Contact ⇀ for further information and inquiries.
